کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1214131 | 1494138 | 2012 | 6 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Determination of 20(S)-protopanaxadiol ocotillol type epimers in rat plasma by liquid chromatography tandem mass spectrometry Determination of 20(S)-protopanaxadiol ocotillol type epimers in rat plasma by liquid chromatography tandem mass spectrometry](/preview/png/1214131.png)
To study stereoselectivity in the pharmacokinetics of each epimer, a rapid, specific and reliable liquid chromatography tandem mass spectrometry method has been established for simultaneous quantitation of both epimers in rat plasma. Plasma samples were pretreated by liquid-liquid extraction. Chromatographic separations were performed on a Shim-pack XR-ODS C18 column (50 mm × 2.1 mm, i.d., 2.2 μm) with an isocratic elution. Both epimers and the internal standard tanshinone II A were ionized with an ESI source operated in positive ion mode and measured by selective reactions monitoring mode. Calibration curve was linear over the concentration range of 1–1000 ng/mL with the lower limit of quantitation of 1 ng/mL for both epimers. Intra and inter-day precisions were less than 6.7% and 9.5%, and the accuracy was within ±5.8% for both epimers. The validated method has been successfully applied to a pharmacokinetic study of the two epimers in rats after oral administration.
► We synthesis the 20(S)-protopanaxadiol ocotillol type epimers.
► We developed a LC–MS/MS method to simultaneously quantitate of two epimers.
► The method was applied to study pharmacokinetics of two epimers.
► We found the stereoselectivity in pharmacokinetics of two epimers was different.
► The study could initially interpret pharmacological effect differences of two epimers.
Journal: Journal of Chromatography B - Volumes 887–888, 1 March 2012, Pages 19–24