کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1214148 1494138 2012 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Determination of geniposidic acid in rat plasma by LC–MS/MS and its application to in vivo pharmacokinetic studies
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Determination of geniposidic acid in rat plasma by LC–MS/MS and its application to in vivo pharmacokinetic studies
چکیده انگلیسی

A simple, rapid and sensitive method for the determination of geniposidic acid (GSA) in rat plasma was developed using liquid chromatography tandem mass spectrometry (LC–MS/MS). Geniposide (GS) was used as the internal standard. Rat plasma pretreated by solid-phase extraction (SPE) was analyzed by LC–MS/MS with negative ion mode electrospray ionization (ESI) in the multiple reaction monitoring (MRM) mode. The analytical column was C8 column and the mobile phase was methanol (A) and water (B). The flow rate was set at 0.8 mL/min with split ratio of 1:3, the total run time was 15 min. The MS/MS ion transitions monitored were m/z 373.3–211.1 for GSA and m/z 387.3–225.3 for GS. The quantification limit was 5 ng/mL within a linear range of 10–4000 ng/mL. The inter-day and intra-day accuracy and precision were within ±10%. The method was fully validated for its sensitivity, selectivity, matrix effect, stability study and recovery. The data indicate that our LC–MS/MS assay is an effective method for the pharmacokinetics study of GSA in rat plasma.


► We firstly developed a LC–MS/MS method for determination of GSA in biological matrix.
► We firstly reported the pharmacokinetic profile of GSA in rats.
► We find that good extraction recovery of GSA was found only using the PXC SPE cartridges.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography B - Volumes 887–888, 1 March 2012, Pages 138–142
نویسندگان
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