کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1214177 | 1494154 | 2010 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Quantification of endogenous steroids in human urine by gas chromatography mass spectrometry using a surrogate analyte approach
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Quantification of endogenous steroids in human urine by gas chromatography mass spectrometry using a surrogate analyte approach Quantification of endogenous steroids in human urine by gas chromatography mass spectrometry using a surrogate analyte approach](/preview/png/1214177.png)
چکیده انگلیسی
Providing “real blank sample” is a problem in determination of endogenous steroids in complex matrices. A new quantification strategy is proposed in the present study, which is based on using isotope-labeled steroids instead of natural steroids for constructing calibration line. This approach is called surrogate analyte and it is shown that its accuracy is better than some of the previously described methods at low concentrations and comparable to standard addition method at medium and high concentration levels. The method was fully validated to satisfy the ICH criteria and it was applied for determination of endogenous steroids in several urine samples.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography B - Volume 878, Issues 11–12, 1 April 2010, Pages 845–852
Journal: Journal of Chromatography B - Volume 878, Issues 11–12, 1 April 2010, Pages 845–852
نویسندگان
Reza Ahmadkhaniha, Abbas Shafiee, Noushin Rastkari, Mohammad Reza Khoshayand, Farzad Kobarfard,