کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1214231 966926 2011 14 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Use of Marfey's reagent and analogs for chiral amino acid analysis: Assessment and applications to natural products and biological systems
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Use of Marfey's reagent and analogs for chiral amino acid analysis: Assessment and applications to natural products and biological systems
چکیده انگلیسی

The present paper describes an updated knowledge and status on Marfey's reagent (MR), 1-fluoro-2,4-dinitrophenyl-5-l-alanine amide (FDNP-l-Ala-NH2). The reagent is used for pre-column derivatization of amino acids followed by HPLC separation of the diastereomers so formed. Emphasis is put on the design and application of structural variants which are synthesized by introducing different (other than l-Ala-NH2) l- and d-amino acid amides and amino acids in the 1,5-difluoro-2,4-dinitro benzene (DFDNB) moiety, as the chiral auxiliary. Advantages, disadvantages, the required precautions and suitability of the approach for the separation of multi component mixtures of dl-amino acids are assessed. Use of two dimensional (2D) techniques, in particular online HPLC in combination with various mass spectrometry techniques is discussed as well as methods designated ‘advanced Marfey's method’ and ‘C3 Marfey's method’. Application of MR and its variants for the determination of the stereochemistry of protein and non-protein amino acids in bioactive natural products isolated from living organisms (bacteria including blue-green algae, filamentous fungi, plants, marine sponges, invertebrates and vertebrates), in physiological samples including human beings, and in biologically relevant synthetic peptides are presented. In an outlook future applications are envisaged.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography B - Volume 879, Issue 29, 1 November 2011, Pages 3148–3161
نویسندگان
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