کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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1214275 | 966927 | 2009 | 6 صفحه PDF | دانلود رایگان |
We report the development of an analytical method combining cell membrane chromatography (CMC) with gas chromatography/mass spectrometry (GC/MS). This was applied to the purification and identification of anti-inflammatory components from traditional Chinese medicines. The stationary phase of the CMC employed mouse peritoneal macrophage (PM) cell membranes. We investigated the performance of the PM/CMC-offline-GC/MS method using hydrocortisone (HC) and dexamethasone (DM) as standards. The method was then applied to the identification of anti-inflammatory components in extracts of Rhizoma Atractylodes macrocephala (RAM) and Rhizoma Atractylodes lancea Thunb DC (RALD). The major components from both species retained by CMC were identified as atractylenolide I (AO-I) by GC/MS. Competition experiments’ results showed that AO-I and lipopolysaccharide (LPS) bound competitively to cell surface receptors while AO-I and HC had only partly overlapping binding sites on the PM membrane. In vitro experiments revealed that AO-I was able to inhibit LPS-induction of TNF-α, IL-1β and NO production in a dose-dependent manner. IC50 values were 5.3 μg/mL, 5.1 μg/mL and 7.5 μg/mL, respectively. The PM/CMC-offline-GC/MS method is an effective screening system for the rapid detection, enrichment, and identification of target components from complex samples.
Journal: Journal of Chromatography B - Volume 877, Issue 27, 1 October 2009, Pages 3019–3024