کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1214369 1494155 2010 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Even-numbered peptides from a papain hydrolysate of silk fibroin
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Even-numbered peptides from a papain hydrolysate of silk fibroin
چکیده انگلیسی

A protease with broad substrate specificity usually produces a complex peptide mixture. However, even-numbered peptides were obtained at high proportion upon papain hydrolysis of fibroin composed of highly repetitive Ala- and Gly-rich blocks. MALDI-TOF and ESI mass spectrometric analysis revealed that the even-numbered peptides were in the forms of di-, tetra-, hexa-, and octa-peptides with repeating units in combination of Ala–Gly, Ser–Gly, Tyr–Gly, and Val–Gly. Application of tandem mass spectrometry identified the sequences of the tetra-peptides to be in the order of Ala–Gly–X–Gly (X = Tyr or Val). Therefore, the substrate specificity of papain and the unique repetitive sequence of fibroin generated the hydrolysate composed of even number of amino acids at a high percentage. In this work, fibroin hydrolysate was investigated as an example of an end product of protein hydrolysis, which provides a clue to understand the fate of peptides in a protein hydrolysate.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography B - Volume 878, Issues 9–10, 15 March 2010, Pages 836–840
نویسندگان
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