کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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1214470 | 966934 | 2011 | 6 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Identification of potassium dehydroandrographolidi succinas and its major metabolites in rat urine by liquid chromatography–tandem mass spectrometry Identification of potassium dehydroandrographolidi succinas and its major metabolites in rat urine by liquid chromatography–tandem mass spectrometry](/preview/png/1214470.png)
A rapid, sensitive and specific liquid chromatography–tandem mass spectrometry (LC–MS/MS) method has been developed for identification of potassium dehydroandrographolidi succinas and its metabolites in rat urine. Five male rats were administrated a single dose (100 mg/kg) of potassium dehydroandrographolidi succinas by i.v. injection. The urine were sampled from 0 to 24 h and purified by using Oasis® HLB extraction cartridge, then the purified urine samples were separated on a reversed-phase C18 column with a linear gradient and detected by an on-line MS detector. Identification and structural elucidation of the metabolites were performed by comparing their changes in molecular mass (Δm) and MS/MS spectra with those of the parent drug. Seven metabolites and the parent drug were found in rat urine. All these metabolites were reported for the first time.
Journal: Journal of Chromatography B - Volume 879, Issue 22, 15 July 2011, Pages 2107–2112