کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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1214478 | 966934 | 2011 | 9 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Determination of carboplatin in human plasma using HybridSPE-precipitation along with liquid chromatography–tandem mass spectrometry Determination of carboplatin in human plasma using HybridSPE-precipitation along with liquid chromatography–tandem mass spectrometry](/preview/png/1214478.png)
The main purpose of this study was to develop and validate a rapid, specific, sensitive, and reliable LC–MS/MS-based bioanalytical method for the determination of carboplatin in human plasma. The optimal chromatographic behavior of carboplatin was achieved on a Biobasic SCX column (50 mm × 2.1 mm, 5 μm) using ion exchange chromatography. The total LC analysis time per injection was 2.6 min with a flow rate of 1.5 mL/min with a gradient elution. Optimization with regard to improving recovery and minimizing matrix effects using HybridSPE-precipitation (HybridSPE-PPT) has been evaluated under various extraction conditions. As a result, sample preparation via HybridSPE-PPT with 1% formic acid in acetonitrile in a 96-well format was applied for method validation and sample analysis and showed acceptable recovery of greater than 25% and negligible matrix effects. The method validation was conducted over the curve range of 2.00–2000 ng/mL using 0.0500 mL of plasma sample. The intra- and inter-day precision and accuracy of the quality control samples at low, medium, and high concentration levels showed ≤4.8% relative standard deviation (RSD) and −13.2 to −3.6% relative errors (RE). The method was successfully applied to determine carboplatin in human plasma samples.
Journal: Journal of Chromatography B - Volume 879, Issue 22, 15 July 2011, Pages 2162–2170