کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1214839 1494043 2016 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Simultaneous determination of phosphatidylcholine-derived quaternary ammonium compounds by a LC–MS/MS method in human blood plasma, serum and urine samples
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Simultaneous determination of phosphatidylcholine-derived quaternary ammonium compounds by a LC–MS/MS method in human blood plasma, serum and urine samples
چکیده انگلیسی


• Simultaneous and fast detection of phosphatidylcholine-derived metabolites.
• Applicable for EDTA-plasma, serum and urine samples.
• High accuracy and precision.

The determination of circulating trimethylamine-N-oxide (TMAO), choline, betaine, l-carnitine and O-acetyl-l-carnitine concentration in different human matrices is of great clinical interest. Recent results highlighted the prognostic value of TMAO and quaternary ammonium containing metabolites in the field of cardiovascular and kidney diseases. Herein, we report a method for the rapid and simultaneous measurement of closely related phosphatidylcholine-derived metabolites in three different biological matrices by stable isotope dilution assay. Plasma, serum and urine samples were simply deproteinized and separated by HILIC-chromatography. Detection and quantification were performed using LC–MS/MS with electrospray ionization in positive mode. For accuracy and precision, full calibration was performed covering more than the full reference range. Assay performance metrics include intra- and interday imprecision were below 10% for all analytes. To exclude matrix effects standard addition methods were applied for all matrices. It was shown that calibration standards and quality control prepared in water can be used instead of matrix-matched calibration and controls. The LC/MS/MS-based assay described in this article may improve future clinical studies evaluating TMAO and related substances as prognostic markers for cardiovascular risk and all-cause mortality in different patient populations.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography B - Volume 1008, 1 January 2016, Pages 206–211
نویسندگان
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