کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1214861 1645350 2009 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
A simple and sensitive HPLC–ESI-MS/MS method for the determination of andrographolide in human plasma
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
A simple and sensitive HPLC–ESI-MS/MS method for the determination of andrographolide in human plasma
چکیده انگلیسی

A liquid chromatography–electrospray ionization tandem mass spectrometry (HPLC–ESI-MS/MS) method for the determination of andrographolide in human plasma was established. Dehydroandrographolide was used as the internal standard (I.S.). The plasma samples were deproteinized with methanol and separated on a Hanbon C18 column with a mobile phase of methanol–water (70:30, v/v). HPLC–ESI-MS/MS was performed in the selected ion monitoring (SIM) mode using target ions at [M−H2O–H]−, m/z 331.1 for andrographolide and [M−H]−, m/z 331.1 for the I.S. Calibration curve was linear over the range of 1.0–150.0 ng/mL. The chromatographic separation was achieved in less than 6.5 min. The lower limits of quantification (LLOQ) was 1.0 ng/mL. The intra and inter-run precisions were less than 6.95 and 7.22%, respectively. The method was successfully applied to determine the plasma concentrations of andrographolide in Chinese volunteers.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography B - Volume 877, Issues 5–6, 15 February 2009, Pages 502–506
نویسندگان
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