کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1214918 | 1494050 | 2015 | 5 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Simultaneous determination of subutinib and its active metabolite in human plasma by LC-MS/MS: Application to pharmacokinetic study
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موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
A selective liquid chromatographic-mass spectrometric method (LC-MS/MS) has been established and validated for simultaneous determination of subutinib and its active metabolite in human plasma. Plasma samples were extracted by liquid-liquid extraction with ethyl acetate and separated on a Wondasil C18 (150 mm Ã 2.1 mm, 3.5 μm), with methanol-0.2% formic acid solution (73:27, v/v) as mobile phase at flow rate of 0.2 ml/min. The linear range was 0.25-100 ng/mL for subutinib and 0.125-50.0 ng/mL for its active metabolite, with lower limit of quantitation of 0.25 ng/mL and 0.125 ng/mL, respectively. Intra- and inter-run precision were within 7.0 and 13.1%, and the accuracies (relative errors) were < 7.0 and 8.0%, with the extraction recoveries 97.0-101.2% and 93.0-98.1% for the two analytes, respectively. The validated method was successfully applied to a pharmacokinetic study of subutinib and its active metabolite in human after oral administration of subutinib maleate capsules.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography B - Volume 1001, 15 September 2015, Pages 22-26
Journal: Journal of Chromatography B - Volume 1001, 15 September 2015, Pages 22-26
نویسندگان
Ding Li-kun, Yang Lin, Chen Su-ning., Li Jian-Kang, Wen Ai-dong,