کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1215178 966965 2008 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Searching biomarker candidates in serum using multidimensional native chromatography: I. Enhanced separation method
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Searching biomarker candidates in serum using multidimensional native chromatography: I. Enhanced separation method
چکیده انگلیسی

The microplate-based method developed by our group for non-denaturing multidimensional proteome separation was improved on using improved column arrays and a newly developed robot. Currently size exclusion, anion exchange and lectin affinity chromatography are combined orthogonally. Different samples run simultaneously to enhance reliability of intercomparison. LC–ESI (electro-spray ionization) MS/MS analysis of selected fractions identified 32,288 peptides matching 2669 serum proteins. The present contribution (I) shows the characteristics of the method, whereas “prove of principle” by applying it to search for biomarker candidates with model diseases is reported in an accompanying paper (II).

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography B - Volume 875, Issue 2, 15 November 2008, Pages 567–572
نویسندگان
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