کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1215273 | 1494180 | 2007 | 6 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: HPLC method for determination of in vitro delivery through and into porcine skin of adefovir (PMEA) HPLC method for determination of in vitro delivery through and into porcine skin of adefovir (PMEA)](/preview/png/1215273.png)
A simple HPLC/UV method for the determination of the transdermal permeation and dermal penetration of a broad-spectrum antiviral drug adefovir (PMEA) was developed. The separation was achieved on a C18 column with the mobile phase composed of 10 mM KH2PO4 and 2 mM Bu4NHSO4 at pH 6.0 and 7% acetonitrile. The method was validated with respect to selectivity, linearity (0.1–50 μg/ml), precision, accuracy, and stability. Transdermal permeation of 2% PMEA was studied in vitro using the Franz diffusion cell and porcine skin. The flux values were 1.8, 3.0, and 0.6 μg/cm2/h from aqueous donor samples at pH 3.4 and 7.4, and isopropyl myristate, respectively. The respective skin concentrations at 48 h were 294, 263, and 971 μg/g from these vehicles. These results will serve as a lead for further studies on transdermal and topical delivery of antivirals from the group of acyclic nucleoside phosphonates.
Journal: Journal of Chromatography B - Volume 853, Issues 1–2, 15 June 2007, Pages 198–203