کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1215338 | 966972 | 2007 | 8 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: cis-3,4-Methylene-heptanoylcarnitine: Characterization and verification of the C8:1 acylcarnitine in human urine cis-3,4-Methylene-heptanoylcarnitine: Characterization and verification of the C8:1 acylcarnitine in human urine](/preview/png/1215338.png)
Acylcarnitine profiles have been used to diagnose specific inherited metabolic diseases. For some acylcarnitines, however, the detailed structure of their acyl group remains a question. One such incompletely characterized acylcarnitine is cis-3,4-methylene-heptanoylcarnitine. To investigate this problem, we isolated the “C8:1” acylcarnitine from human urine, transesterified it to form its acyl picolinyl ester, and characterized it by GC/EI-MS. These results were compared to GC/EI-MS results from authentic standards we synthesized (cis-3,4-methylene-heptanoylcarnitine, trans-2-octenoylcarnitine, 3-octenoylcarnitine, cis-4-octenoylcarnitine, and trans-4-octenoylcarnitine). Only cis-3,4-methylene-heptanoylcarnitine matched the urinary “C8:1” acylcarnitine. The standards were then spiked in human urine, converted to pentafluorophenacyl esters, and detected by HPLC/MS. cis-3,4-Methylene-heptanoylcarnitine exactly matched the “C8:1” acylcarnitine in urine, whereas none of the other C8:1 acylcarnitine standards matched. Based on the data from GC/EI-MS and HPLC/MS, the “C8:1” acylcarnitine in human urine is shown to be cis-3,4-methylene-heptanoylcarnitine.
Journal: Journal of Chromatography B - Volume 857, Issue 2, 1 October 2007, Pages 251–258