کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1215500 966975 2008 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Fast and direct quantification of adrenal steroids by tandem mass spectrometry in serum and dried blood spots
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Fast and direct quantification of adrenal steroids by tandem mass spectrometry in serum and dried blood spots
چکیده انگلیسی

We present a fast and reproducible method for steroid analysis (corticosterone, deoxycorticosterone, progesterone, 17α-hydroxyprogesterone, 11-deoxycortisol, 21-deoxycortisol, androstenedione, testosterone, dihydrotestosterone and cortisol) in small volumes of serum and in dried blood spot samples by LC-MS/MS. No derivatisation was needed. LC separation was achieved by using an Atlantis® C18 column and water–methanol–formic acid gradient as a mobile phase and a flow rate of 250 μL/min over a run time of 6 min. Steroids were measured in MRM mode with electrospray interface (positive ion mode). Validation showed excellent precision, sensitivity, recovery and linearity with coefficients of determination r2 > 0.992.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography B - Volume 861, Issue 1, 1 January 2008, Pages 117–122
نویسندگان
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