کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1215556 | 966977 | 2007 | 5 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Rapid quantitation of testosterone hydroxyl metabolites by ultra-performance liquid chromatography and mass spectrometry Rapid quantitation of testosterone hydroxyl metabolites by ultra-performance liquid chromatography and mass spectrometry](/preview/png/1215556.png)
A rapid and sensitive ultra-performance liquid chromatography and mass spectrometry (UPLC/MS) method was developed to simultaneously quantify seven monohydroxyl testosterone metabolites (16α-, 2α-, 7α-, 6α-, 2β-, 6β-, and 16β-hydroxyl testosterones) in rat liver microsomes. The UPLC system used a short 1.7-μm particle size column coupled to a Sciex 4000 Q trap in multiple reaction monitor (MRM) mode. All hydroxyl testosterones were resolved within 2.5 min. A 4-day validation was performed to determine the linearity, repeatability, reproducibility and accuracy of the method in rat liver microsomes. This method is applicable to the measurement of the testosterone hydroxylase activity in biological matrices such as the liver microsome incubates.
Journal: Journal of Chromatography B - Volume 855, Issue 2, 15 August 2007, Pages 290–294