کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1215600 | 1494183 | 2007 | 11 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Simultaneous profiling of N-glycans and proteins from human serum using a parallel-column system directly coupled to mass spectrometry
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
A method for the rapid identification of proteins and their N-glycans was developed through the use of two parallel columns directly connected to a mass spectrometer. Both porous graphitic carbon (PGC) and C18 capillary columns were connected in parallel with two switching valves for the simultaneous analysis of glycans and peptides, respectively. To verify the efficiency of the analytical system, profiling of N-glycans and proteins from human serum was demonstrated. This method is suitable for high-throughput analysis and automation, is contamination-free for the identification of N-glycans and proteins in a complex biological sample, and can be applied to glycomics and proteomics.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography B - Volume 850, Issues 1â2, 1 May 2007, Pages 109-119
Journal: Journal of Chromatography B - Volume 850, Issues 1â2, 1 May 2007, Pages 109-119
نویسندگان
Yun-Gon Kim, Kyoung-Soon Jang, Hwang-Soo Joo, Hyun-Ki Kim, Chang-Soo Lee, Byung-Gee Kim,