کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1215669 | 1494176 | 2007 | 8 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Comparison of ESI-MS interfaces for the analysis of UV-crosslinked peptide–nucleic acid complexes Comparison of ESI-MS interfaces for the analysis of UV-crosslinked peptide–nucleic acid complexes](/preview/png/1215669.png)
In this report, the effectiveness of high performance liquid chromatography (HPLC) in conjunction with electrospray ionization mass spectrometry (ESI-MS) is examined as a tool for identifying the sites of crosslinking in a protein that has been photoreacted with a non-photolabeled oligonucleotide. ESI-MS and MALDI-MS analyses preceded by off-line microflow and nanoflow HPLC, on-line microflow HPLC/ESI, and on-line nanoflow HPLC/ESI interfaces were performed in order to determine their relative effectiveness in separating mixtures of nucleopeptides and identifying sites of crosslinking on the individual components. The characteristics of these four techniques as well as possibilities for improving the analysis of nucleopeptides by ESI-MS are compared and discussed.
Journal: Journal of Chromatography B - Volume 860, Issue 2, 15 December 2007, Pages 145–152