کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1215880 1494077 2014 14 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Annotation of the human serum metabolome by coupling three liquid chromatography methods to high-resolution mass spectrometry
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Annotation of the human serum metabolome by coupling three liquid chromatography methods to high-resolution mass spectrometry
چکیده انگلیسی


• We have evaluated three LC/HRMS methods for their coverage of human serum metabolome.
• The three platforms appeared complementary (less than 12% of common annotations).
• HILIC column ensured the greatest metabolome coverage in the negative ionization mode.
• PFPP column ensured the greatest metabolome coverage in the positive ionization mode.
• 266 metabolites were identified in serum, and 59 are reported for the first time.

This work aims at evaluating the relevance and versatility of liquid chromatography coupled to high resolution mass spectrometry (LC/HRMS) for performing a qualitative and comprehensive study of the human serum metabolome. To this end, three different chromatographic systems based on a reversed phase (RP), hydrophilic interaction chromatography (HILIC) and a pentafluorophenylpropyl (PFPP) stationary phase were used, with detection in both positive and negative electrospray modes. LC/HRMS platforms were first assessed for their ability to detect, retain and separate 657 metabolite standards representative of the chemical families occurring in biological fluids. More than 75% were efficiently retained in either one LC-condition and less than 5% were exclusively retained by the RP column. These three LC/HRMS systems were then evaluated for their coverage of serum metabolome. The combination of RP, HILIC and PFPP based LC/HRMS methods resulted in the annotation of about 1328 features in the negative ionization mode, and 1358 in the positive ionization mode on the basis of their accurate mass and precise retention time in at least one chromatographic condition. Less than 12% of these annotations were shared by the three LC systems, which highlights their complementarity. HILIC column ensured the greatest metabolome coverage in the negative ionization mode, whereas PFPP column was the most effective in the positive ionization mode. Altogether, 192 annotations were confirmed using our spectral database and 74 others by performing MS/MS experiments. This resulted in the formal or putative identification of 266 metabolites, among which 59 are reported for the first time in human serum.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography B - Volume 966, 1 September 2014, Pages 34–47
نویسندگان
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