کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1216139 1494161 2009 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Determination of p-phenylenediamine and its metabolites MAPPD and DAPPD in biological samples using HPLC-DAD and amperometric detection
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Determination of p-phenylenediamine and its metabolites MAPPD and DAPPD in biological samples using HPLC-DAD and amperometric detection
چکیده انگلیسی

A sensitive and selective HPLC method using a diode array detector (DAD) and an electrochemical detector (ECD) in series has been developed and validated for the quantitative measurement of p-phenylenediamine and its acetylated metabolites N-acetyl-p-phenylenediamine (MAPPD) and N,N′-diacetyl-p-phenylenediamine (DAPPD) in biological samples. The separation was carried out on a hydrophilic modified AQUA C18 column and the mobile phase was composed of acetonitrile: ammonium acetate solution (5:95, 25 mM, v/v). Spectrophotometric detection was performed at 240 or 255 nm and amperometric detection was carried out using a positive oxidation potential of 400 mV. The quantification of the three analytes was validated in the range of 0.05–50 μM and the established limits of determination were 0.5 μM for PPD and MAPPD and 1 μM for DAPPD. The standard deviations (N = 9) were lower than 7.5% at a concentration of 1 μM. The samples were stabilised with ascorbic acid to prevent PPD from oxidizing. Pretreatment of samples or analyte enrichment before sample injection is not required. The method proved to be accurate, sensitive and sufficiently specific. It was applied to the ecotoxicological study of the kinetics of the PPD N-acetylation in cell lysates in two different media.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography B - Volume 877, Issues 16–17, 1 June 2009, Pages 1627–1633
نویسندگان
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