کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1216281 1494092 2014 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Investigation of volatile organic metabolites in lung cancer pleural effusions by solid-phase microextraction and gas chromatography/mass spectrometry
ترجمه فارسی عنوان
بررسی متابولیت های آلی فرار در پلورال افیوژن های سرطانی ریه توسط میکرو اکستراکتیو فاز جامد و طیف سنجی کروماتوگرافی گاز
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
چکیده انگلیسی


• Volatile organic metabolites were found in pleural effusions by HS-SPME-GC/MS.
• Five compounds exhibited significant difference between the two groups.
• High levels of cyclehexanone and 2-ethyl-1-hexanol in malignant effusions.
• Two compounds might be candidate biomarkers of malignant effusions.
• Pleural effusion is a valuable sample source for observation of changes in VOMs.

Headspace solid-phase microextraction (HS-SPME) combined with gas chromatography/mass spectrometry (GC/MS) method was applied for the investigation of low molecular weight volatile organic metabolites (VOMs) in pleural effusion samples. Three important HS-SPME experimental parameters that influence extraction efficiency (fiber coating, extraction time and temperature of sampling) were optimized by a univariate optimization design. The highest extraction efficiency was obtained when sampling was performed at 50 °C for 10 min under agitation using a carboxen/polydimethylsiloxane (CAR/PDMS) fiber. A total of 36 volatile metabolites belonging to nine distinct chemical classes were identified in 40 pleural effusion samples (20 malignant effusions from lung cancer patients and 20 benign effusions from inflammatory patients). Ketones, alcohols, and benzene derivatives were the main chemical classes for the metabolomic profile of malignant effusions. The average peak areas of ketones and alcohols were much higher in malignant group compared to benign group. Together with phenols, they exhibit significant differences (P < 0.05) between the two groups. Particularly, the average peak areas of cyclohexanone and 2-ethyl-1-hexanol in malignant effusions were significantly higher than those in benign ones. Furthermore, of the 36 identified metabolites, 5 compounds including cyclohexanone and 2-ethyl-1-hexanol were found to be statistically different (Student's t-test, P < 0.05) between the two groups by statistical analysis based on the peak areas of all identified metabolites. Among them, cyclohexanone and 2-ethyl-1-hexanol might be considered as candidate biomarkers of lung cancer to differentiate malignant from benign effusions. The results show that HS-SPME-GC/MS is a simple, rapid, sensitive and solvent-free method for the determination of VOMs in pleural effusion samples. Pleural effusion is a valuable sample source for observation of changes in VOMs for differentiation between lung cancer patients and inflammatory individuals.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography B - Volumes 945–946, 15 January 2014, Pages 53–59
نویسندگان
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