کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1216557 1494188 2006 4 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Development of an HPLC method for the determination of nifedipine in human plasma by solid-phase extraction
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Development of an HPLC method for the determination of nifedipine in human plasma by solid-phase extraction
چکیده انگلیسی

Nifedipine, a dihydropyridine calcium channel antagonist, is widely used in the treatment of hypertension and other cardiovascular disorders. A selective, sensitive and accurate high-performance liquid chromatographic method has been developed, validated and applied for determination of nifedipine in human plasma samples. A series of studies were conducted in order to investigate the effects of mobile phase composition, buffer concentration, mobile phase pH and concentration of organic modifiers, and to develop a convenient and easy-to-use method for quantitative analysis of nifedipine. The method involves solid-phase extraction on C18 cartridges. The chromatographic separation was accomplished on a Lichrocart Lichrospher 60 RP selectB column with a mobile phase composed of 0.020 mol/L KH2PO4 (pH 4.8) and acetonitrile (42:58, v/v). UV detection was set at 240 nm. The calibration curve was linear in the concentration range of 5.0–200.0 ng/mL for nifedipine in plasma and the limit of quantification was 5.0 ng/mL.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography B - Volume 839, Issues 1–2, 24 July 2006, Pages 85–88
نویسندگان
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