کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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1216669 | 1494192 | 2006 | 8 صفحه PDF | دانلود رایگان |

A fast and selective HPLC-MS–MS method was established to determine l-threonate in human plasma and urine. Plasma and urine samples were extracted by protein precipitation and diluted with water, then chromatographed on an YMC J'Sphere C18 column with methanol–acetonitrile–10 mM ammonium acetate (20:5:75, v/v) as mobile phase, and at a flow rate of 0.2 ml/min. Detection was performed on a triple–quadrupole tandem mass spectrometer using negative electrospray ionization (ESI). Multiple reactions monitoring (MRM) was used and l-threonate was quantified by monitoring the ion transition of m/z 134.5 → 74.7. The linear calibration curves of l-threonate in plasma and urine were obtained over the concentration range of 0.25–50 μg/ml and 2.5–500 μg/ml, respectively. Lower limit of quantitation was 0.25 and 2.5 μg/ml, respectively. Accuracy was within 85–115%, and intra- and inter-batch precision (R.S.D.%) were within ±15%. The method proved to be accurate and specific, and was applied to the pharmacokinetic study of l-threonate in Chinese healthy subjects.
Journal: Journal of Chromatography B - Volume 834, Issues 1–2, 13 April 2006, Pages 155–162