کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1216672 1494192 2006 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Determination of spectinomycin hydrochloride and its related substances by HPLC–ELSD and HPLC–MSn
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Determination of spectinomycin hydrochloride and its related substances by HPLC–ELSD and HPLC–MSn
چکیده انگلیسی

A new and simple high-performance liquid chromatography–evaporative light scattering detection (HPLC–ELSD) method for the determination of spectinomycin hydrochloride and its related substances was developed. The column was Agilent SB-C18 (250 mm × 4.6 mm, 5 μm).The mobile phase was 25 mM trifluoroacetic acid. The drift tube temperature was 40 °C. The pressure of nebulizing gas was 3.5 bar. Good separation of spectinomycin from main related substances could be achieved. The standard curve was rectilinear in the range of 0.07–3.8 mg/ml (r = 0.9997). Precision was 1.0% (R.S.D.). The limit of detection was 6 μg/ml. The method is simple and rapid, and the results are accurate and reproducible. The HPLC–MSn method was used to characterize the structures of impurities contained in the spectinomycin. In positive mode, impurities were elucidated by use of electrospray ion trap mass spectrometry in the multi-stage MS full scan mode. The possible structures of impurities C and D in spectinomycin were deduced based on the HPLC–MSn data.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography B - Volume 834, Issues 1–2, 13 April 2006, Pages 178–182
نویسندگان
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