Antibody affinity purification using metallic nickel particles

► Solid nickel ferromagnetic particles for rapid, scalable, and efficient antibody affinity purification. ► Immobilizing Protein A on the surface of nickel particles for chromatographic and diagnostic applications. ► Minimizing Protein A leaching during antibody affinity purification. ► Isolating IgG antibodies directly from mouse serum in less than 5 min. ► Magnetic separation methods combining the pre-cleaning step and the affinity capture into one single step.