کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1217029 1494134 2012 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Hollow fiber liquid-phase microextraction combined with high performance liquid chromatography for the determination of trace mitiglinide in biological fluids
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Hollow fiber liquid-phase microextraction combined with high performance liquid chromatography for the determination of trace mitiglinide in biological fluids
چکیده انگلیسی

A hollow fiber liquid phase microextraction (HF-LPME) in conjunction with reversed phase HPLC–UV method was developed for the extraction and determination of trace amounts of the antidiabetic drug, mitiglinide (MIT) in biological fluids. The drug was extracted from 10 mL aqueous sample (donor phase (DP)) into an organic phase impregnated in the pores of hollow fiber, followed by the back extraction into a second aqueous solution (acceptor phase (AP)) located in the lumen of the hollow fiber. Parameters influencing the extraction efficiency including the kind of organic solvent, composition of DP and AP, extraction time, stirring rate and salt addition were investigated and optimized. Under the optimized extraction conditions, high enrichment factors (210-fold), good linearity (5–1000 ng mL−1) and detection limit lower than 1.38 ng mL−1 were achieved. Recoveries of spiked samples were in the range (88.3–96.3%) and (92.0–99.3%) for urine and plasma samples, respectively. The percent relative standard deviation (n = 9) for the extraction and determination of three concentration levels (100, 400 and 800 ng mL−1) of MIT were less than 10.6% and 13.6% for urine and plasma samples, respectively. The developed method is simple, sensitive and has been successfully applied to the analysis of MIT in biological fluids.


► HF-LPME was for the first time reported for extraction of MIT in biological fluids.
► After the extraction, MIT was analyzed using HPLC–UV.
► The sample clean-up offered clean extracts.
► Resulting in significant reduction in analysis time.
► The proposed method is fast, sensitive, consumes minute amounts of extracting solvent.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography B - Volumes 895–896, 1 May 2012, Pages 131–136
نویسندگان
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