کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1217032 1494134 2012 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Simultaneous determination of six alkaloids and one monoterpene in rat plasma by liquid chromatography–tandem mass spectrometry and pharmacokinetic study after oral administration of a Chinese medicine Wuji Pill
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Simultaneous determination of six alkaloids and one monoterpene in rat plasma by liquid chromatography–tandem mass spectrometry and pharmacokinetic study after oral administration of a Chinese medicine Wuji Pill
چکیده انگلیسی

A simple and sensitive method for simultaneous determination of seven active constituents, jatrorrhizine, berberine, coptisine, palmatine, evodiamine, rutacarpine and paeoniflorin, from a Chinese medicine Wuji Pill in rat plasma was developed based on a liquid chromatography and tandem mass spectrometry method. The separation of these seven compounds was carried out on a Shiseido CAPCELL PAK C18 column using a mobile phase consisting of acetonitrile (containing 0.1% formic acid and water (containing 0.1% formic acid and 10 mmol/L ammonium acetate) and carbamazepine as an internal standard. Electrospray ionization in positive-ion mode and multiple reaction monitoring was used to identify and quantitate active components. All calibration curves gave good linearity (r > 0.993) over the concentration range from 0.42–208.0 ng/mL to 4.18–418.0 ng/mL for all components. The precision of the in vivo study was evaluated by intra- and inter-day assays and the percentages of relative standard deviation were all within 15%. The method was successfully applied to pharmacokinetic study of all six alkaloids and one monoterpene in rat plasma after oral administration of the Wuji Pill.


► LC–MS/MS method for simultaneous quantitation of six alkaloids and one monterpene.
► Seven active constituents of “Wuji Pill” simultaneously quantitated in rat plasma.
► LLOQs of these seven constituents are below 4.18 ng/mL.
► Efficient one-step liquid–liquid extraction of these constituents in rat plasma.
► Pharmacokinetic of seven compounds in rat plasma after oral dosing of “Wuji Pill”.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography B - Volumes 895–896, 1 May 2012, Pages 154–161
نویسندگان
, , , , , , , , ,