کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1217108 1494177 2007 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Determination of duloxetine in human plasma by liquid chromatography with atmospheric pressure ionization–tandem mass spectrometry and its application to pharmacokinetic study
کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Determination of duloxetine in human plasma by liquid chromatography with atmospheric pressure ionization–tandem mass spectrometry and its application to pharmacokinetic study
چکیده انگلیسی

A rapid, sensitive and accurate liquid chromatographic–tandem mass spectrometry (LC–MS–MS) method is described for the determination of duloxetine in human plasma. Duloxetine was extracted from plasma using methanol and separated on a C18 column. The mobile phase consisting of a mixture of acetonitrile and 5 mM ammonium acetate (45:55, v/v, pH 3.5) was delivered at a flow rate of 0.3 ml/min. Atmospheric pressure ionization (API) source was operated in positive ion mode. Multiple reaction monitoring (MRM) mode using the transitions of m/z 298.1 → m/z 44.0 and m/z 376.2 → m/z 123.2 were used to quantify duloxetine and internal standard (I.S.), respectively. The linearity was obtained over the concentration range of 0.1–50.0 ng/ml and the lower limit of quantitation (LLOQ) was 0.1 ng/ml. This method was successfully applied to pharmacokinetic study of a duloxetine formulation product after oral administration to healthy human subjects.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography B - Volume 858, Issues 1–2, 15 October 2007, Pages 269–275
نویسندگان
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