کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1217430 1494193 2006 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Quantitative determination of atractylenolide III in rat plasma by liquid chromatography electrospray ionization mass spectrometry
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Quantitative determination of atractylenolide III in rat plasma by liquid chromatography electrospray ionization mass spectrometry
چکیده انگلیسی

Atractylenolide III is a major active component in Atractylodes macrocephala. This paper describes a simple, rapid, specific and sensitive method for the quantification of atractylenolide III in rat plasma using a liquid–liquid extraction procedure followed by liquid chromatography mass spectrometric (LC-MS) analysis. A Kromasil 3.5 μm C18 column (150 mm × 2.00 mm) was used as the analytical column. Linear detection responses were obtained for atractylenolide III concentration ranging from 5 to 500 ng mL−1. The precision and accuracy data, based on intra-day and inter-day variations over 5 days were within 10.29%. The lower limit of quantitation for atractylenolide III was 5 ng mL−1, using 0.1 mL plasma for extraction and its recoveries were greater than 85% at the low, medium and high concentrations. The method has been successfully applied to a pharmacokinetic study in rats after an oral administration of atractylenolide III with a dose of 20.0 mg kg−1. With the lower limits of quantification at 5 ng mL−1 for atractylenolide III, this method was proved to be sensitive enough for the pharmacokinetics study of atractylenolide III.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography B - Volume 831, Issues 1–2, 2 February 2006, Pages 36–41
نویسندگان
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