کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1217556 | 967112 | 2009 | 5 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Development and validation of a LC/MS/MS method for quantification of nobiliside A in rat plasma
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موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Development and validation of a LC/MS/MS method for quantification of nobiliside A in rat plasma Development and validation of a LC/MS/MS method for quantification of nobiliside A in rat plasma](/preview/png/1217556.png)
چکیده انگلیسی
A LC/MS/MS method was developed and validated for determination of nobiliside A in rat plasma. Analyses were separated by an Agella Venusil ASB-C18 column and isocratic elution with methanol:water (80:20, v/v) as a mobile phase. A tandem mass spectrometer was used as a detector for quantitative analysis. Calibration curves (R2 > 0.999) in spiked plasma were linear over the concentration range of 50–5000 ng/mL. The overall accuracy of this method was 93–104% for nobiliside A. Within-day and between-day variability was both less than 9% in plasma. The data indicate that the LC/MS/MS method is an effective method for the pharmacokinetics study of nobiliside A in rat plasma.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography B - Volume 877, Issue 3, 15 January 2009, Pages 323–327
Journal: Journal of Chromatography B - Volume 877, Issue 3, 15 January 2009, Pages 323–327
نویسندگان
Dan Guo, Yang Xiong, Yue Zhang, Zhongbin Wu, Lili Cui, Jianming Chen,