کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1217585 1494167 2008 4 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Solvent-enhanced microwave-assisted derivatization following solid-phase extraction combined with gas chromatography–mass spectrometry for determination of amphetamines in urine
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Solvent-enhanced microwave-assisted derivatization following solid-phase extraction combined with gas chromatography–mass spectrometry for determination of amphetamines in urine
چکیده انگلیسی

An approach using microwave-assisted derivatization (MAD) following solid-phase extraction (SPE) combined with gas chromatography–mass spectrometry (GC–MS) was developed to determine amphetamines in urine samples. The parameters affecting the derivatization efficiency – including microwave power and irradiation time – were investigated. Besides, solvent is thought critically important to MAD. Derivatization performance was studied using various solvents and compared with the performance obtained without solvent. Derivatization efficiency was clearly found to be enhanced by the presence of solvent. The highest derivatization efficiencies were obtained in ethyl acetate (EA) under microwave power of 250 W for 1 min. Calibration curves for all amphetamines were linear over a range from 1 to 1000 ng/mL, with correlation coefficients above 0.9992. The intra-day and inter-day precision were less than 15%. The applicability of the method was tested by analyzing amphetamine-abusing subjects urine samples. Accordingly, the solvent-enhanced MAD-GC–MS method appears to be adequate for determining amphetamines in urine.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography B - Volume 874, Issues 1–2, 15 October 2008, Pages 115–118
نویسندگان
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