کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1217712 | 1494178 | 2007 | 5 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
High performance liquid chromatographic measurement of iothalamate in human serum and urine for evaluation of glomerular filtration rate
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: High performance liquid chromatographic measurement of iothalamate in human serum and urine for evaluation of glomerular filtration rate High performance liquid chromatographic measurement of iothalamate in human serum and urine for evaluation of glomerular filtration rate](/preview/png/1217712.png)
چکیده انگلیسی
A simple and sensitive HPLC-UV assay was developed for the measurement of iothalamate (IOT) in human serum and urine. Chromatographic separation was achieved using an embedded-carbamate-group bonded RP18 column and mobile phase consisting of 50 mM monobasic sodium phosphate and methanol (90:10, v/v) without the addition of ion-pair reagents. The assay demonstrated a high analytical reliability within the IOT concentration range of 1-150 μg/ml in serum and 25-1500 μg/ml in urine. The relative standard deviations (RSDs) for intra- and inter-day analysis were less than 5.1% in all cases. This method has been used for the evaluation of glomerular filtration rate (GFR) in subjects participating in a phase I clinical trial of a novel antimalarial medicine. The average baseline GFR was 100.41 ± 19.99 ml/min/1.73 m2 in 119 healthy volunteers. The assay may also allow the simultaneous measurements of p-aminohippuric acid (PAH), N-acetyl PAH (aPAH), and IOT with some modification. PAH, IOT, aPAH, and β-hydroxyethyl-theophylline internal standard peaks appeared approximately at 2.5, 3.7, 5.9, and 11.8 min, respectively, in an isocratic run.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography B - Volume 856, Issues 1â2, 1 September 2007, Pages 95-99
Journal: Journal of Chromatography B - Volume 856, Issues 1â2, 1 September 2007, Pages 95-99
نویسندگان
Daoqin Bi, Kevin J. Leary, Julie A. Weitz, Svetlana A. Cherstniakova, Michael A. Reil, Michael J. Roy, Louis R. Cantilena,