کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1217787 | 1494179 | 2007 | 5 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Validated HPLC method for the determination of gabapentin in human plasma using pre-column derivatization with 1-fluoro-2,4-dinitrobenzene and its application to a pharmacokinetic study Validated HPLC method for the determination of gabapentin in human plasma using pre-column derivatization with 1-fluoro-2,4-dinitrobenzene and its application to a pharmacokinetic study](/preview/png/1217787.png)
A rapid, sensitive and accurate high-performance liquid chromatographic method with UV detection was developed and validated for the quantification of gabapentin in human plasma. Gabapentin was quantified using pre-column derivatization with 1-fluoro-2,4-dinitrobenzene following protein precipitation of plasma with acetonitrile. Amlodipine was used as internal standard. The chromatographic separation was carried out on a Nova-Pak C18 column using a mixture of 50 mM NaH2PO4 (pH = 2.5)–acetonitrile (30:70, v/v) as mobile phase with UV detection at 360 nm. The flow rate was set at 1.5 ml/min. The method was linear over the range of 0.05–5 μg/ml of gabapentin in plasma (r2 > 0.999). The within-day and between-day precision values were in the range of 2–5%. The limit of quantification of the method was 0.05 μg/ml. The method was successfully used to study the pharmacokinetics of gabapentin in healthy volunteers.
Journal: Journal of Chromatography B - Volume 854, Issues 1–2, 1 July 2007, Pages 43–47