کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1217860 | 1494184 | 2007 | 11 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Preparation of membrane proteins for analysis by two-dimensional gel electrophoresis
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Preparation of membrane proteins for analysis by two-dimensional gel electrophoresis Preparation of membrane proteins for analysis by two-dimensional gel electrophoresis](/preview/png/1217860.png)
چکیده انگلیسی
In order to separate hydrophobic membrane proteins, we have developed a novel two-dimensional electrophoresis system. For the iso-electric focusing, agarose was used as a supporting matrix and n-dodecyl-β-d-maltopyranoside was used as a surfactant. In combination with a previously developed Tris/MES electrophoresis system in the second dimension, distinct spots were reproducibly detected from hydrophobic membrane proteins whose grand average hydropathicity (GRAVY) exceed 0.3. In contrast to the immobilized pH gradient system, c-type heme was also visualized in this system.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography B - Volume 849, Issues 1–2, 15 April 2007, Pages 282–292
Journal: Journal of Chromatography B - Volume 849, Issues 1–2, 15 April 2007, Pages 282–292
نویسندگان
Yasuhiro Kashino, Takeshi Harayama, Himadri B. Pakrasi, Kazuhiko Satoh,