کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1217916 | 967167 | 2007 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Validation of an HIV-1 inactivation protocol that is compatible with intracellular drug analysis by mass spectrometry
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Validation of an HIV-1 inactivation protocol that is compatible with intracellular drug analysis by mass spectrometry Validation of an HIV-1 inactivation protocol that is compatible with intracellular drug analysis by mass spectrometry](/preview/png/1217916.png)
چکیده انگلیسی
Mass spectrometry is a powerful tool for studying the intracellular pharmacokinetics of antiretroviral drugs. However, the biohazard of HIV-1 calls for a safety protocol for such analyses. To this end, we extracted HIV-1 producing cells with methanol or ethanol at 4 °C. After extraction, no viral infectivity was detected, as shown by a reduction in infectious titers of more than 6 log. In addition, this protocol is compatible with the quantitative analysis of antiretroviral drugs in cell extracts using matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) MS. Thus, using this protocol, infectious HIV-1 is inactivated and antiretroviral drugs are extracted from cells in a single step.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography B - Volume 847, Issue 1, 15 February 2007, Pages 38–44
Journal: Journal of Chromatography B - Volume 847, Issue 1, 15 February 2007, Pages 38–44
نویسندگان
Jeroen J.A. van Kampen, Esther J. Verschuren, Peter C. Burgers, Theo M. Luider, Ronald de Groot, Albert D.M.E. Osterhaus, Rob A. Gruters,