کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1225254 | 1494741 | 2016 | 19 صفحه PDF | دانلود رایگان |
• The review presents and discusses protocols for depletion of abundant serum proteins.
• It also presents approaches for the effective analysis of whole, untreated serum.
• Next it elaborates on multiple molecular forms/multiple functions of serum proteins.
• It then recommends that information on abundant serum proteins be routinely obtained.
The first sections of this review compile and discuss strategies and protocols for managing plasma/serum as a source of biomarkers relevant to human disease. In many such cases, depletion of abundant protein(s) is a crucial preliminary step to the procedure; specific conceptual and technical approaches, however, make it possible to effectively use to this purpose whole plasma/serum. The final sections focus instead on the complexity associated with each of the major serum/plasma proteins in terms of both, multiple molecular structures (existence of a number of protein species) and of multiple molecular functions (behavior as multifunctional/multitasking/moonlighting proteins). Reviewing evidence in these and some related fields (regulation of the synthetic pattern by proteins and non-protein compounds and its connection with health and disease) prompts the suggestion/recommendation that information on the abundant components of plasma/serum proteome is routinely obtained and processed/mined as a valuable contribution to the characterization of any non-physiological condition and to the understanding of its mechanisms and of its implications/sequels.
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Journal: Journal of Proteomics - Volume 140, 17 May 2016, Pages 62–80