Evidence for a new post-translational modification in Staphylococcus aureus: Hydroxymethylation of asparagine and glutamine

Staphylococcus aureus is an opportunistic pathogen whose infectious capacity depends on surface proteins, which enable bacteria to colonize and invade host tissues and cells. We analyzed “trypsin-shaved” surface proteins of S. aureus cultures by high resolution LC-MS/MS at different growth stages and culture conditions. Some modified peptides were identified, with a mass shift corresponding to the addition of a CH2O group (+ 30.0106 u). We present evidence that this shift corresponds to a hyxdroxymethylation of asparagine and glutamine residues. This known but poorly documented post-translational modification was only found in a few proteins of S. aureus grown under specific conditions. This specificity seemed to exclude the hypothesis of an artifact due to sample preparation. Altogether hydroxymethylation was observed in 35 peptides from 15 proteins in our dataset, which corresponded to 41 modified sites, 35 of them being univocally localized. While no function can currently be assigned to this post-translational modification, we hypothesize that it could be linked to modulation of virulence factors, since it was mostly found on some surface proteins of S. aureus.

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► Infectious capacity of Staphylococcus aureus depends on surface proteins.
► We analyzed trypsin-shaved surface proteins of S.aureus at different growth stages.
► Hydroxymethylation of asparagine or glutamine was detected with high resolution MS.
► The modification was observed in 41 sites of 35 peptides from 15 proteins.
► Our data confirm the existence of this PTM, supported by scarce evidence until now.