کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1225513 1494754 2015 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Heat differentiated complement factor profiling
ترجمه فارسی عنوان
پروفیل فاکتور مکمل حرارت متمایز شده است
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
چکیده انگلیسی


• We present a multiplex immunoassay applicable for complement deficiency screening.
• A preanalytical heat treatment was found to influence the measurements.
• Sera from 16 patients with complement deficiency were profiled with the assay.
• Dried blood spots from patients were processed successfully.
• The presented assay enables neonatal screening for complement deficiency.

Complement components and their cascade of reactions are important defense mechanisms within both innate and adaptive immunity. Many complement deficient patients still remain undiagnosed because of a lack of high throughput screening tools. Aiming towards neonatal proteome screening for immunodeficiencies, we used a multiplex profiling approach with antibody bead arrays to measure 9 complement proteins in serum and dried blood spots. Several complement components have been described as heat sensitive, thus their heat-dependent detectability was investigated. Using sera from 16 patients with complement deficiencies and 23 controls, we confirmed that the proteins C1q, C2, C3, C6, C9 and factor H were positively affected by heating, thus the identification of deficient patients was improved when preheating samples. Measurements of C7, C8 and factor I were negatively affected by heating and non-heated samples should be used in analysis of these components. In addition, a proof of concept study demonstrated the feasibility of labeling eluates from dried blood spots to perform a subsequent correct classification of C2-deficiencies. Our study demonstrates the potential of using multiplexed single binder assays for screening of complement components that open possibilities to expand such analysis to other forms of deficiencies.

Figure optionsDownload high-quality image (306 K)Download as PowerPoint slide

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Proteomics - Volume 126, 3 August 2015, Pages 155–162
نویسندگان
, , , , , , , , ,