کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1225525 1494754 2015 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Beyond single modification: Reanalysis of the acetylproteome of human sperm reveals widespread multiple modifications
ترجمه فارسی عنوان
فراتر از اصلاح تک: بازآزمایی استیل پروتئوم اسپرم انسان نشان می دهد تعدادی از تغییرات گسترده
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
چکیده انگلیسی


• We evaluated the results of reanalysis with three variable modifications.
• Ubiquitinated peptides could be co-enriched with acetylated peptides.
• Phosphorylated peptides are randomly eluted with acetylated peptides.
• About 500 proteins contain more than one type of PTM in human sperm.

Sperm is an ideal model for studying post-translational modifications since its transcriptional and translational activities are nearly silent. Thus, sperm functions are mainly regulated at the protein level, especially by means of post-translational modifications. Published proteomic datasets may contain valuable undiscovered information. In this study, we reanalyzed the raw data from previous acetylproteome study on human capacitated sperm to include two additional modifications: phosphorylation and ubiquitination. We successfully identified approximately 500 proteins with multiple types of modifications. Compared with recently developed serial enrichment strategy for multiple modifications, reanalysis of single modification enriched data provides a direct and efficient alternative approach. These results greatly expand our knowledge of protein modifications in human sperm.

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ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Proteomics - Volume 126, 3 August 2015, Pages 296–302
نویسندگان
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