کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1225580 1494802 2013 10 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Comparative vesicle proteomics reveals selective regulation of protein expression in chestnut blight fungus by a hypovirus
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Comparative vesicle proteomics reveals selective regulation of protein expression in chestnut blight fungus by a hypovirus
چکیده انگلیسی

The chestnut blight fungus (Cryphonectria parasitica) and hypovirus constitute a model system to study fungal pathogenesis and mycovirus–host interaction. Knowledge in this field has been gained largely from investigations at gene transcription level so far. Here we report a systematic analysis of the vesicle proteins of the host fungus with/without hypovirus infection. Thirty-three differentially expressed protein spots were identified in the purified vesicle protein samples by two-dimensional electrophoresis and mass spectrometry. Down-regulated proteins were mostly cargo proteins involved in primary metabolism and energy generation and up-regulated proteins were mostly vesicle associated proteins and ABC transporter. A virus-encoded protein p48 was found to have four forms with different molecular mass in vesicles from the virus-infected strain. While a few of the randomly selected differentially expressed proteins were in accordance with their transcription profiles, majority were not in agreement with their mRNA accumulation patterns, suggesting that an extensive post-transcriptional regulation may have occurred in the host fungus upon a hypovirus infection.

Figure optionsDownload high-quality image (63 K)Download as PowerPoint slideHighlights
► This is the first report employing 2-DE/MS technology to study vesicle proteome of the chestnut blight fungus Cryphonectria parasitica.
► Hypovirus infection selectively regulates host protein expression in vesicles of C. parasitica.
► Isoforms derived from a viral protein p48 were discovered.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Proteomics - Volume 78, 14 January 2013, Pages 221–230
نویسندگان
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