A new bioadhesive material from fish parasite Neobenedenia girellae

• A new adhesive material from N. girellae is characterized by de novo sequencing.
• The main components of the glue are cytoskeletal proteins.
• The study gives new insights on parasite adhesion to commercially relevant fish.

The purpose of the work was to identify the proteins present in the adhesive material of the capsalid Neobenedenia girellae by a proteomic approach based on de novo sequencing and data base search to overcome the lack of information concerning the genome of these parasites. Glandular secretions were obtained by a new method, set up in our laboratory, which allowed collecting a small amount of secretion without any contamination from other tissues either from the parasites as well as from the skin of the host. The proteomic analysis reveals that the adhesive is mainly composed of cytoskeletal proteins (actin, keratin and tubulin) but contains also ATP-synthase, 78 kDa glucose regulated protein and albumin.Biological significanceThis paper reports for the first time the characterization of a novel bioadhesive material used by capsalid parasites to adhere to fish. Such information broadens our knowledge of the molecular mechanisms involved in adhesiveness of parasites to hosts. Moreover, it offers new clues in understanding the mechanism of stickiness and adhesion of cytoskeleton components, often involved in both physiological and pathological processes, including neurodegenerative diseases.

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