کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1225793 968253 2011 12 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
A workflow for peptide-based proteomics in a poorly sequenced plant: A case study on the plasma membrane proteome of banana
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
A workflow for peptide-based proteomics in a poorly sequenced plant: A case study on the plasma membrane proteome of banana
چکیده انگلیسی

Membrane proteins are an interesting class of proteins because of their functional importance. Unfortunately their analysis is hampered by low abundance and poor solubility in aqueous media. Since shotgun methods are high-throughput and partly overcome these problems, they are preferred for membrane proteomics. However, their application in non-model plants demands special precautions to prevent false positive identification of proteins.In the current paper, a workflow for membrane proteomics in banana, a poorly sequenced plant, is proposed. The main steps of this workflow are (i) optimization of the peptide separation, (ii) performing de novo sequencing to allow a sequence homology search and (iii) visualization of identified peptide–protein associations using Cytoscape to remove redundancy and wrongly assigned peptides, based on species-specific information. By applying this workflow, integral plasma membrane proteins from banana leaves were successfully identified.

Graphical AbstractFigure optionsDownload high-quality image (125 K)Download as PowerPoint slide

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Proteomics - Volume 74, Issue 8, 12 August 2011, Pages 1218–1229
نویسندگان
, , , , , , , ,