کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1225862 968255 2010 16 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Analysis of abscisic acid responsive proteins in Brassica napus guard cells by multiplexed isobaric tagging
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Analysis of abscisic acid responsive proteins in Brassica napus guard cells by multiplexed isobaric tagging
چکیده انگلیسی

Guard cells, which form stomata on the leaf epidermis, play important roles in plant gas exchange and defense against pathogens. Abscisic acid (ABA) is a phytohormone that can be induced by drought and leads to stomatal closure. Guard cells have been a premier model system for studying ABA signal transduction. Despite significant progress on the identification of molecular components in the ABA signaling pathway, our knowledge of the protein components is very limited. Here, we employ a recently developed multiplexed isobaric tagging technology to identify ABA-responsive proteins in Brassica napus guard cells. A total of 431 unique proteins were identified with relative quantitative information in control and ABA-treated samples. Proteins involved in stress and defense constituted a major group among the 66 proteins with increased abundance. Thirty-eight proteins were decreased in abundance and fell into several functional groups including metabolism and protein synthesis. Many of the proteins have not been reported as being ABA responsive or involved in stomatal movement. A large percentage of the protein-coding genes contained ABA-responsive elements. This study not only established a comprehensive inventory of ABA-responsive proteins, but also identified new proteins for further investigation of their functions in guard cell ABA signaling.

Multiplex iTRAQ technology was employed to reveal the ABA-responsive proteins in guard cells and the results were organized into a proposed signaling network worth of further investigation.Figure optionsDownload high-quality image (685 K)Download as PowerPoint slide

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Proteomics - Volume 73, Issue 4, 10 February 2010, Pages 790–805
نویسندگان
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