کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1225934 968263 2011 12 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
An analysis of the Caenorhabditis elegans lipid raft proteome using geLC-MS/MS
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
An analysis of the Caenorhabditis elegans lipid raft proteome using geLC-MS/MS
چکیده انگلیسی

Lipid rafts are microdomains of the phospholipid bilayer, proposed to form semi-stable “islands” that act as a platform for several important cellular processes; major classes of raft-resident proteins include signalling proteins and glycosylphosphatidylinositol (GPI)-anchored proteins. Proteomic studies into lipid rafts have been mainly carried out in mammalian cell lines and single cell organisms. The nematode Caenorhabditis elegans, the model organism with a well-defined developmental profile, is ideally suited for the study of this subcellular locale in a complex developmental context. A study of the lipid raft proteome of C. elegans is presented here. A total of 44 proteins were identified from the lipid raft fraction using geLC-MS/MS, of which 40 have been determined to be likely raft proteins after analysis of predicted functions. Prediction of GPI-anchoring of the proteins found 21 to be potentially modified in this way, two of which were experimentally confirmed to be GPI-anchored. This work is the first reported study of the lipid raft proteome in C. elegans. The results show that raft proteins, including numerous GPI-anchored proteins, may have a variety of potentially important roles within the nematode, and will hopefully lead to C. elegans becoming a useful model for the study of lipid rafts.

Graphical AbstractTo enable the identification of lipid raft proteins in C. elegans, sucrose density gradient centrifugation was used to enrich the detergent -resistant raft membranes. The proteins were separated using gel electrophoresis, digested with trypsin and analysed by LC-MS/MS. Identified proteins were then classified according to function and location using gene ontological tools.Figure optionsDownload high-quality image (70 K)Download as PowerPoint slide

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Proteomics - Volume 74, Issue 2, 1 February 2011, Pages 242–253
نویسندگان
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