کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1225950 968264 2012 16 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Proteomic profiling of rapid non-genomic and concomitant genomic effects of acute restraint stress on rat thymocytes
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Proteomic profiling of rapid non-genomic and concomitant genomic effects of acute restraint stress on rat thymocytes
چکیده انگلیسی

In order to investigate rapid non-genomic effects of acute stress, rats were restrained for 15 min which was sufficient to activate the hypothalamus–pituitary–adrenal (HPA) axis but too short to induce massive genomic effects of cortisol. Subcellular fractions of thymocytes (cytosol, nucleus, membrane) were investigated using quantitative 2D DIGE with MALDI-TOF/TOF mass spectrometry. In total, 108 proteins with differential subcellular localizations were identified. The specificity of the changes induced by psychological stress was reflected by the prominent modulation of proteins involved in the HPA and sympathoadrenal medullar (SAM) axis such as HMGB1 and NHERF1. Intracellular trafficking was characterized by a dominant protein exodus from the cytosol. Real translocation was observed for 9 proteins with 6 that shuttled from the cytosol to the nucleus (HYOU1, HNRPF, HNRPC, STRAP, PSA1, PPA1) and 3 from the nucleus to the cytosol (HMGB1, NHERF1, PSMA1). Proteins showing subcellular reshuffling were largely involved in transcription and translation processes (39 of 108) with a significant enrichment of RNA splicing factors. Bioinformatics analysis revealed significant enrichment for protein kinase A and 14-3-3 signaling, probably reflecting real non-genomic effects. This is the first study investigating rapid effects of stress-induced HPA activation in vivo at the proteome level.

Figure optionsDownload high-quality image (91 K)Download as PowerPoint slideHighlights
► 108 proteins show differential subcellular localization after 15 min acute psychological stress (restraint stress).
► 39 of 108 proteins are involved in transcription/translation with high enrichment for splicing factors (genomic effects).
► Bioinformatics approach shows enrichment for protein kinase A and 14-3-3 signaling (non-genomic effects).
► NUC → CYT: HMGB1 and NHERF1 (interaction partners of GR and ADRB2; signature of stress).

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Proteomics - Volume 75, Issue 7, 3 April 2012, Pages 2064–2079
نویسندگان
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