کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1226047 968270 2008 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Solubilization of membrane protein complexes for blue native PAGE
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Solubilization of membrane protein complexes for blue native PAGE
چکیده انگلیسی

Blue native PAGE is an electrophoretic technique for high-resolution separation of membrane proteins. The method has been proven especially useful for investigation of native protein complexes enabling a characterization of potential protein–protein interactions in the context of functional proteomics. Blue native PAGE is easy to realise, results are reproducible and a high number of protocols are available. However, care should be taken during solubilization of protein complexes to achieve significant results in BN-PAGE analysis. Solubilization of membranes and proteins is not only influenced by detergent–lipid and detergent–protein interactions but also by lipid–lipid, lipid–protein and protein–protein interactions. Interactions have been investigated experimentally and theoretically. But, in practice, the experimental results do not always mirror the theoretical basis and therefore optimal solubilization conditions for each membrane and membrane protein complex should be investigated individually to tap the full potential of BN-PAGE analysis.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Proteomics - Volume 71, Issue 3, 21 August 2008, Pages 277–283
نویسندگان
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