کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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1226050 | 968270 | 2008 | 14 صفحه PDF | دانلود رایگان |
A high-throughput approach for biomolecule analysis is demonstrated for a mixture of peptides from tryptic digest of four proteins as well as a tryptic digests of human plasma. In this method a chip based electrospray autosampler coupled to a hybrid ion mobility (IMS) mass spectrometer (MS) is utilized to achieve rapid sample analysis. This high-throughput measurement is realized by exploiting the direct infusion capability of the chip based electrospray with its rapid sample manipulating capability as well as a high sensitive IMS–MS with a recently developed IMS–IMS separation technique that can be multiplexed to provide greater throughput. From replicate IMS–MS runs of known mixtures, the average uncertainty of peak intensities is determined to be ± 7% (relative standard deviation), and a detection limit in the low attomole range is established. The method is illustrated by analyzing 124 human plasma protein samples in duplicate, a measurement that required 16.5 h. Current limitations as well as implications of the high-throughput approach for complex biological sample analysis are discussed.
Journal: Journal of Proteomics - Volume 71, Issue 3, 21 August 2008, Pages 318–331