Proteomic analysis of salicylic acid induced resistance to Mungbean Yellow Mosaic India Virus in Vigna mungo

The role of salicylic acid (SA) in inducing resistance to MYMIV infection in Vigna mungo has been elucidated by proteomics. Twenty-nine proteins identified by MALDI-TOF/TOF, predicted to be involved in stress responses, metabolism, photosynthesis, transport and signal transduction, showed increased abundance upon SA treatment. Susceptible plants showed characteristic yellow mosaic symptoms upon MYMIV infection. A concentration dependent decrease in physiological symptoms associated with MYMIV was observed upon exogenous SA treatment prior to viral inoculation; and no visible symptom was observed at 100 μM SA. SA treatment stimulated SOD and GPX activity and inhibited CAT activity thus preventing ROS mediated damage. Significant increase in chlorophyll, protein, carbohydrate, phenolic content and H2O2 were observed. Involvement of calmodulin for transmission of defense signal by SA is suggested. A metabolic reprogramming leading to enhanced synthesis of proteins involved in primary and secondary metabolisms is necessary for SA mediated resistance to MYMIV. Identification of proteins showing increased abundance, involved in photosynthetic process is a significant finding which restores virus-induced degradation of the photosynthetic apparatus and provides enhanced metabolites required for repartition of resources towards defense.

Graphical AbstractSA mediated induction of MYMIV-resistance in susceptible Vigna mungo Cv. T9.Figure optionsDownload high-quality image (728 K)Download as PowerPoint slideResearch Highlights
► MYMIV adversely affects V. mungo plants at biochemical and physiological levels.
► SA-treatment decreased disease symptoms in a concentration dependent manner.
► Twenty-nine SA induced proteins were identified by MALDI-TOF MS and MS/MS.
► Induced proteins were stress, metabolism, photosynthesis and transport related.
► Complex metabolic reprogramming provides MYMIV-tolerance to SA treated V. mungo.