کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1226156 968282 2010 11 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Exploring the membrane proteome—Challenges and analytical strategies
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Exploring the membrane proteome—Challenges and analytical strategies
چکیده انگلیسی

The analysis of proteins in biological membranes forms a major challenge in proteomics. Despite continuous improvements and the development of more sensitive analytical methods, the analysis of membrane proteins has always been hampered by their hydrophobic properties and relatively low abundance. In this review, we describe recent successful strategies that have led to in-depth analyses of the membrane proteome. To facilitate membrane proteome analysis, it is essential that biochemical enrichment procedures are combined with special analytical workflows that are all optimized to cope with hydrophobic polypeptides. These include techniques for protein solubilization, and also well-matched developments in protein separation and protein digestion procedures. Finally, we discuss approaches to target membrane–protein complexes and lipid–protein interactions, as such approaches offer unique insights into function and architecture of cellular membranes.

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ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Proteomics - Volume 73, Issue 5, 10 March 2010, Pages 868–878
نویسندگان
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