کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1226213 968285 2009 4 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
A software program for more reliable precursor ion assignation from LC-MS analysis using LTQ ultra zoom scan
کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
A software program for more reliable precursor ion assignation from LC-MS analysis using LTQ ultra zoom scan
چکیده انگلیسی

We developed a software program (titled Precursor Ion Calibration software for LTQ or, in short, PICsL) that increases the reliability of precursor ion assignations from LC-MS analysis using ultra zoom scanning of LTQ linear ion trap MS and automatically corrects the assignations. Although existing software calculates the theoretical isotopic distribution according to m/z with a computational algorithm, our method simply searches for ions close to the theoretical mass value using both MS/MS raw data and Mascot search result files, followed by a second database search that identifies the proteins using the regenerated peak list files. Our software program mimics the manual inspection of the spectral data of precursor ions and is expected to be applicable not only for low resolution MS, such as LTQ, but also for a wide variety of MS instruments.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Proteomics - Volume 73, Issue 2, 1 December 2009, Pages 357–360
نویسندگان
, , , ,